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anti m 6 a antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals anti m 6 a antibody
    Anti M 6 A Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m6a/N6-methyladenosine+(m6A)+Antibody+-+Azide+and+BSA+Free/bio_rxiv__64898__2026__04__04__716518-82-18-22
    Average 92 stars, based on 2 article reviews
    anti m 6 a antibody - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Integrated multi-omics analysis reveals molecular changes associated with chronic lipid accumulation following contusive spinal cord injury.
    Article Snippet: Spinal cord tissue centered at the injury site was embedded in optimal cutting temperature compound (TissueTek, 4583, Sakura) and cut into 20 μm sections using a Leica CM1950 cryostat. .. Sections were rinsed three times in PBS, followed by blocking using 10% normal donkey serum in 0.1% Triton X-100 in 0.01 M PBS buffer for 30 min, then incubated overnight at room temperature with primary antibodies including MBP (1:500, Cell Signaling Technology, 78,896), SMI312 (1:1000, BioLegend, 837,904), SMI32 (1:400, BioLegend, 801,701), Iba1 (1:1000, Abcam, ab283319), GFAP (1:400, Cell Signaling Technology, 80,788), LC3 (1:200, Cell Signaling Technology, 12741S), PFKL (1:100, Abcam, ab181064), F4/80 (1:200, Cell Signaling Technology, 71299S), and m6A (1:200, Novus Biologicals, NBP3–05657). .. The following day, sections were washed three times in PBS, and incubated with donkey anti-mouse Alexa Fluor 488 (1:200, Abcam ab150105), donkey anti-rabbit Alexa Fluor 555 (1:200, Abcam ab150062) and donkey anti-rat Alexa Fluor 647 (1:200, Abcam ab150155) at room temperature for 2 h. For lipid droplets (LDs) and cholesterol ester staining, sections were incubated with BODIPYTM 493/503 (1:400, Invitrogen D3922) or CholEsteryl BODIPYTM 542/563 C11(1:400, Invitrogen, C12680) at room temperature for 20 min, then slices were washed three times in PBS and mounted with Fluoromount-G (0100–20, Southern Biotech).

    Incubation:

    Article Title: Integrated multi-omics analysis reveals molecular changes associated with chronic lipid accumulation following contusive spinal cord injury.
    Article Snippet: Spinal cord tissue centered at the injury site was embedded in optimal cutting temperature compound (TissueTek, 4583, Sakura) and cut into 20 μm sections using a Leica CM1950 cryostat. .. Sections were rinsed three times in PBS, followed by blocking using 10% normal donkey serum in 0.1% Triton X-100 in 0.01 M PBS buffer for 30 min, then incubated overnight at room temperature with primary antibodies including MBP (1:500, Cell Signaling Technology, 78,896), SMI312 (1:1000, BioLegend, 837,904), SMI32 (1:400, BioLegend, 801,701), Iba1 (1:1000, Abcam, ab283319), GFAP (1:400, Cell Signaling Technology, 80,788), LC3 (1:200, Cell Signaling Technology, 12741S), PFKL (1:100, Abcam, ab181064), F4/80 (1:200, Cell Signaling Technology, 71299S), and m6A (1:200, Novus Biologicals, NBP3–05657). .. The following day, sections were washed three times in PBS, and incubated with donkey anti-mouse Alexa Fluor 488 (1:200, Abcam ab150105), donkey anti-rabbit Alexa Fluor 555 (1:200, Abcam ab150062) and donkey anti-rat Alexa Fluor 647 (1:200, Abcam ab150155) at room temperature for 2 h. For lipid droplets (LDs) and cholesterol ester staining, sections were incubated with BODIPYTM 493/503 (1:400, Invitrogen D3922) or CholEsteryl BODIPYTM 542/563 C11(1:400, Invitrogen, C12680) at room temperature for 20 min, then slices were washed three times in PBS and mounted with Fluoromount-G (0100–20, Southern Biotech).



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    Proteintech anti m6a antibody
    NEU1 mRNA stability is regulated by <t>m6A</t> modification. (A) Total m6A levels in RNA from mouse atrial tissues and atrial fibroblasts were measured using an m6A quantification kit. (B) Western blot analysis of METTL3 expression in atrial tissues and atrial fibroblasts. (C) RNA immunoprecipitation (RIP) assay confirming the interaction between NEU1 and METTL3 in atrial fibroblasts. (D) Western blot analysis of METTL3 expression in primary mouse atrial fibroblasts. (E) MeRIP-qPCR assay detecting the m6A modification level on NEU1 mRNA in primary mouse atrial fibroblasts. (F) NEU1 mRNA stability assessed by qPCR in atrial fibroblasts treated with actinomycin D at 0, 3, and 6 hours post-transfection with sh-NC or sh-METTL3.
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    Image Search Results


    Loss of METTL14 alters m6A modification, leading to mitochondrial dysfunction, cGAS-STING-IFN-I activation, and necroptosis. These parallel pathways converge to drive cardiomyopathy and heart failure.

    Journal: bioRxiv

    Article Title: METTL14-dependent m 6 A modification restrains interferon signaling to prevent myocarditis and dilated Cardiomyopathy

    doi: 10.64898/2026.04.02.716218

    Figure Lengend Snippet: Loss of METTL14 alters m6A modification, leading to mitochondrial dysfunction, cGAS-STING-IFN-I activation, and necroptosis. These parallel pathways converge to drive cardiomyopathy and heart failure.

    Article Snippet: Membranes were then blocked with 5% nonfat dry milk in 1× PBST for 1 h at room temperature and incubated overnight at 4 °C with an anti-m6A antibody (1:5,000; Cell Signaling Technology, Cat. #56593).

    Techniques: Modification, Activation Assay

    NEU1 mRNA stability is regulated by m6A modification. (A) Total m6A levels in RNA from mouse atrial tissues and atrial fibroblasts were measured using an m6A quantification kit. (B) Western blot analysis of METTL3 expression in atrial tissues and atrial fibroblasts. (C) RNA immunoprecipitation (RIP) assay confirming the interaction between NEU1 and METTL3 in atrial fibroblasts. (D) Western blot analysis of METTL3 expression in primary mouse atrial fibroblasts. (E) MeRIP-qPCR assay detecting the m6A modification level on NEU1 mRNA in primary mouse atrial fibroblasts. (F) NEU1 mRNA stability assessed by qPCR in atrial fibroblasts treated with actinomycin D at 0, 3, and 6 hours post-transfection with sh-NC or sh-METTL3.

    Journal: Cell Adhesion & Migration

    Article Title: N6-methyladenosine modification of NEU1 mediated by METTL3 exacerbates angiotensin II-induced atrial fibrillation

    doi: 10.1080/19336918.2026.2650272

    Figure Lengend Snippet: NEU1 mRNA stability is regulated by m6A modification. (A) Total m6A levels in RNA from mouse atrial tissues and atrial fibroblasts were measured using an m6A quantification kit. (B) Western blot analysis of METTL3 expression in atrial tissues and atrial fibroblasts. (C) RNA immunoprecipitation (RIP) assay confirming the interaction between NEU1 and METTL3 in atrial fibroblasts. (D) Western blot analysis of METTL3 expression in primary mouse atrial fibroblasts. (E) MeRIP-qPCR assay detecting the m6A modification level on NEU1 mRNA in primary mouse atrial fibroblasts. (F) NEU1 mRNA stability assessed by qPCR in atrial fibroblasts treated with actinomycin D at 0, 3, and 6 hours post-transfection with sh-NC or sh-METTL3.

    Article Snippet: For each sample, 50 μg of fragmented RNA was reserved as input, and the remaining RNA was incubated with 5 μg of anti-m6A antibody (68055–1-Ig, Proteintech) or IgG antibody (30000–0-AP, Proteintech) at 4°C overnight with gentle rotation, followed by incubation with Protein A/G magnetic beads (88802, Thermo Fisher Scientific) for 2 hours at 4°C.

    Techniques: Modification, Western Blot, Expressing, RNA Immunoprecipitation, Transfection